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Comparative cell wall thickness and growth of VISA isolates. ( A ) Transmission electron microscopy (TEM) of representative VISA clinical isolates and VSSA control strains. The yellow bar indicates 1 µm. ( B ) Cell wall thickness (nm) was measured manually by using TEM images with ImageJ software as described in Materials and Methods. Results are the mean ± standard deviation of 30 cells (5–10 measurements per cell). Data were analyzed by using a one-way analysis of variance and <t>Dunnett’s</t> posttest. * P < 0.05 versus the relevant VSSA control strain for each CC (N315 for CC5, LAC for CC8, and CA-347 for CC45). ( C ) Effect of vancomycin on the growth rate of VISA clinical isolates (22 CC5 isolates, 24 CC8 isolates, and 27 CC45 isolates). Bacteria were cultured in BHI broth with 0 or 2 µg/mL of vancomycin. The colored bar indicates variance in growth rate among the isolates (i.e., time required to reach an OD 600 of 0.2). The vertical black line indicates median time of growth for each group.
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Comparative cell wall thickness and growth of VISA isolates. ( A ) Transmission electron microscopy (TEM) of representative VISA clinical isolates and VSSA control strains. The yellow bar indicates 1 µm. ( B ) Cell wall thickness (nm) was measured manually by using TEM images with ImageJ software as described in Materials and Methods. Results are the mean ± standard deviation of 30 cells (5–10 measurements per cell). Data were analyzed by using a one-way analysis of variance and <t>Dunnett’s</t> posttest. * P < 0.05 versus the relevant VSSA control strain for each CC (N315 for CC5, LAC for CC8, and CA-347 for CC45). ( C ) Effect of vancomycin on the growth rate of VISA clinical isolates (22 CC5 isolates, 24 CC8 isolates, and 27 CC45 isolates). Bacteria were cultured in BHI broth with 0 or 2 µg/mL of vancomycin. The colored bar indicates variance in growth rate among the isolates (i.e., time required to reach an OD 600 of 0.2). The vertical black line indicates median time of growth for each group.
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Comparative cell wall thickness and growth of VISA isolates. ( A ) Transmission electron microscopy (TEM) of representative VISA clinical isolates and VSSA control strains. The yellow bar indicates 1 µm. ( B ) Cell wall thickness (nm) was measured manually by using TEM images with ImageJ software as described in Materials and Methods. Results are the mean ± standard deviation of 30 cells (5–10 measurements per cell). Data were analyzed by using a one-way analysis of variance and <t>Dunnett’s</t> posttest. * P < 0.05 versus the relevant VSSA control strain for each CC (N315 for CC5, LAC for CC8, and CA-347 for CC45). ( C ) Effect of vancomycin on the growth rate of VISA clinical isolates (22 CC5 isolates, 24 CC8 isolates, and 27 CC45 isolates). Bacteria were cultured in BHI broth with 0 or 2 µg/mL of vancomycin. The colored bar indicates variance in growth rate among the isolates (i.e., time required to reach an OD 600 of 0.2). The vertical black line indicates median time of growth for each group.
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Comparative cell wall thickness and growth of VISA isolates. ( A ) Transmission electron microscopy (TEM) of representative VISA clinical isolates and VSSA control strains. The yellow bar indicates 1 µm. ( B ) Cell wall thickness (nm) was measured manually by using TEM images with ImageJ software as described in Materials and Methods. Results are the mean ± standard deviation of 30 cells (5–10 measurements per cell). Data were analyzed by using a one-way analysis of variance and <t>Dunnett’s</t> posttest. * P < 0.05 versus the relevant VSSA control strain for each CC (N315 for CC5, LAC for CC8, and CA-347 for CC45). ( C ) Effect of vancomycin on the growth rate of VISA clinical isolates (22 CC5 isolates, 24 CC8 isolates, and 27 CC45 isolates). Bacteria were cultured in BHI broth with 0 or 2 µg/mL of vancomycin. The colored bar indicates variance in growth rate among the isolates (i.e., time required to reach an OD 600 of 0.2). The vertical black line indicates median time of growth for each group.
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Comparative cell wall thickness and growth of VISA isolates. ( A ) Transmission electron microscopy (TEM) of representative VISA clinical isolates and VSSA control strains. The yellow bar indicates 1 µm. ( B ) Cell wall thickness (nm) was measured manually by using TEM images with ImageJ software as described in Materials and Methods. Results are the mean ± standard deviation of 30 cells (5–10 measurements per cell). Data were analyzed by using a one-way analysis of variance and <t>Dunnett’s</t> posttest. * P < 0.05 versus the relevant VSSA control strain for each CC (N315 for CC5, LAC for CC8, and CA-347 for CC45). ( C ) Effect of vancomycin on the growth rate of VISA clinical isolates (22 CC5 isolates, 24 CC8 isolates, and 27 CC45 isolates). Bacteria were cultured in BHI broth with 0 or 2 µg/mL of vancomycin. The colored bar indicates variance in growth rate among the isolates (i.e., time required to reach an OD 600 of 0.2). The vertical black line indicates median time of growth for each group.
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GraphPad Software Inc one-way analysis of variance with newman-keuls multiple comparison post test graphpad prism version 4.0 for windows
Comparative cell wall thickness and growth of VISA isolates. ( A ) Transmission electron microscopy (TEM) of representative VISA clinical isolates and VSSA control strains. The yellow bar indicates 1 µm. ( B ) Cell wall thickness (nm) was measured manually by using TEM images with ImageJ software as described in Materials and Methods. Results are the mean ± standard deviation of 30 cells (5–10 measurements per cell). Data were analyzed by using a one-way analysis of variance and <t>Dunnett’s</t> posttest. * P < 0.05 versus the relevant VSSA control strain for each CC (N315 for CC5, LAC for CC8, and CA-347 for CC45). ( C ) Effect of vancomycin on the growth rate of VISA clinical isolates (22 CC5 isolates, 24 CC8 isolates, and 27 CC45 isolates). Bacteria were cultured in BHI broth with 0 or 2 µg/mL of vancomycin. The colored bar indicates variance in growth rate among the isolates (i.e., time required to reach an OD 600 of 0.2). The vertical black line indicates median time of growth for each group.
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Comparative cell wall thickness and growth of VISA isolates. ( A ) Transmission electron microscopy (TEM) of representative VISA clinical isolates and VSSA control strains. The yellow bar indicates 1 µm. ( B ) Cell wall thickness (nm) was measured manually by using TEM images with ImageJ software as described in Materials and Methods. Results are the mean ± standard deviation of 30 cells (5–10 measurements per cell). Data were analyzed by using a one-way analysis of variance and <t>Dunnett’s</t> posttest. * P < 0.05 versus the relevant VSSA control strain for each CC (N315 for CC5, LAC for CC8, and CA-347 for CC45). ( C ) Effect of vancomycin on the growth rate of VISA clinical isolates (22 CC5 isolates, 24 CC8 isolates, and 27 CC45 isolates). Bacteria were cultured in BHI broth with 0 or 2 µg/mL of vancomycin. The colored bar indicates variance in growth rate among the isolates (i.e., time required to reach an OD 600 of 0.2). The vertical black line indicates median time of growth for each group.
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Comparative cell wall thickness and growth of VISA isolates. ( A ) Transmission electron microscopy (TEM) of representative VISA clinical isolates and VSSA control strains. The yellow bar indicates 1 µm. ( B ) Cell wall thickness (nm) was measured manually by using TEM images with ImageJ software as described in Materials and Methods. Results are the mean ± standard deviation of 30 cells (5–10 measurements per cell). Data were analyzed by using a one-way analysis of variance and <t>Dunnett’s</t> posttest. * P < 0.05 versus the relevant VSSA control strain for each CC (N315 for CC5, LAC for CC8, and CA-347 for CC45). ( C ) Effect of vancomycin on the growth rate of VISA clinical isolates (22 CC5 isolates, 24 CC8 isolates, and 27 CC45 isolates). Bacteria were cultured in BHI broth with 0 or 2 µg/mL of vancomycin. The colored bar indicates variance in growth rate among the isolates (i.e., time required to reach an OD 600 of 0.2). The vertical black line indicates median time of growth for each group.
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Comparative cell wall thickness and growth of VISA isolates. ( A ) Transmission electron microscopy (TEM) of representative VISA clinical isolates and VSSA control strains. The yellow bar indicates 1 µm. ( B ) Cell wall thickness (nm) was measured manually by using TEM images with ImageJ software as described in Materials and Methods. Results are the mean ± standard deviation of 30 cells (5–10 measurements per cell). Data were analyzed by using a one-way analysis of variance and Dunnett’s posttest. * P < 0.05 versus the relevant VSSA control strain for each CC (N315 for CC5, LAC for CC8, and CA-347 for CC45). ( C ) Effect of vancomycin on the growth rate of VISA clinical isolates (22 CC5 isolates, 24 CC8 isolates, and 27 CC45 isolates). Bacteria were cultured in BHI broth with 0 or 2 µg/mL of vancomycin. The colored bar indicates variance in growth rate among the isolates (i.e., time required to reach an OD 600 of 0.2). The vertical black line indicates median time of growth for each group.

Journal: Microbiology Spectrum

Article Title: Insights into the molecular basis of reduced vancomycin susceptibility among three prominent Staphylococcus aureus clonal complexes

doi: 10.1128/spectrum.00486-24

Figure Lengend Snippet: Comparative cell wall thickness and growth of VISA isolates. ( A ) Transmission electron microscopy (TEM) of representative VISA clinical isolates and VSSA control strains. The yellow bar indicates 1 µm. ( B ) Cell wall thickness (nm) was measured manually by using TEM images with ImageJ software as described in Materials and Methods. Results are the mean ± standard deviation of 30 cells (5–10 measurements per cell). Data were analyzed by using a one-way analysis of variance and Dunnett’s posttest. * P < 0.05 versus the relevant VSSA control strain for each CC (N315 for CC5, LAC for CC8, and CA-347 for CC45). ( C ) Effect of vancomycin on the growth rate of VISA clinical isolates (22 CC5 isolates, 24 CC8 isolates, and 27 CC45 isolates). Bacteria were cultured in BHI broth with 0 or 2 µg/mL of vancomycin. The colored bar indicates variance in growth rate among the isolates (i.e., time required to reach an OD 600 of 0.2). The vertical black line indicates median time of growth for each group.

Article Snippet: Data for each VISA CC were compared to the VSSA control strain by using a one-way analysis of variance and Dunnett’s posttest (GraphPad Prism version 10.1.0, GraphPad Software, Boston, MA, USA).

Techniques: Transmission Assay, Electron Microscopy, Control, Software, Standard Deviation, Bacteria, Cell Culture